Effect of acid hydrolases in the regressing tail of Rana tigrina
Keywords:
investigation, specific, phenomenonAbstract
Progressive metamorphosis includes epidermal cells, spinal cord, muscle fibers and connective tissue along with tail reabsorption. It is unclear how resorption proceeds and to what extent the signals for the death process are transmitted between cells. We determined the kinetics of metamorphosis, apoptosis, and tail regression in the diploid anuran, Rana tigrina. In the present investigation acid phosphatase activity (μMole Pi.hr-1.mg-1of fresh wt.) was found to be increased from 9*10-4 to 26*10-3 in the tail homogenae of tadpole larva of Rana tigrina from stage III to stage XXI (Table 3). In this connection specific activity (μMole Pi.hr-1.mg-1of protein) of acid phosphatase measured at different development stages, there was a 4-fold increase in stage XVIII and 6-fold increase in stage XXI. Alkaline phosphatase activity stood at 5.95 KA units on stage III of tadpole larva underwent a near 2-fold increase in stage XXI. Enzyme activity in the trunk was also found to be slightly increased from 6.6KA unit at stage (III-XVIII) to 7.68KA unit at stage (XIX-XXI). Some authors even reported the decreased level of alkaline phosphatase after a few days from start of climax stage in the tadpole larva. This suggested that the excretion phenomenon was limited to the bulk discharge of the whole lysosome content analogous to that which occurs when lysosomes are submitted in vitro to activating treatments.References
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