PHYTOCHEMICAL SCREENING FOR SECONDARY METABOLITES IN AN ENDANGERED MEDICINAL PLANT GLORIOSA SUPERBA L.

Authors

  • K.Geetha, G.Jansi Rani , B, Sugashini Mrs.A.P.Gowsalya Devi, Department of botany with specialization in Plant biotechnology, The Standard fireworks Rajaratnam college for Women, Sivakasi Assistant Professor of Botany. The Standard fireworks Rajaratnam college for Women, Sivakasi

Keywords:

Endangered medicinal plant, Gloriosa superb L., secondary metabolites, Phytochemicals.

Abstract

Gloriosa superba L. has been a source of medicine right from ancient time. Medicinal plants have bioactive compounds which are used for curing of various human diseases and also play an important role in healing. Screening of phytochemical constituents using different organic solvents (low polar to high polar) in the leaves were studied. The leaves of the selected medicinal plants were washed, air dried and then powdered. The aqueous extract of leaf samples were used for the phytochemical analysis to find out the phytochemical constituents.The main objective was to check the presence or absence of the phytochemical constituents in the leaves of the selected medicinal plant. The results of the phytochemical analysis showed that the alkaloids, saponin, tannins and terpenoids were highly present in various solvent extracts of Gloriosa superba L. Among these extracts ethanol and acetone extract showed high level of phytochemical properties. Indiscriminate removal, difficulty in vegetative propagation and poor germination accounts for the depletion of useful plant species. Plant tissue culture techniques are used as an alternative method for the production of specific metabolites and also for the propagation of plant species at a large scale. In the present study preliminary screening for the presence of secondary metabolites was studied in order to understand the levels of phytochemicals, so that in vitro production of secondary metabolites using cell cultures will be initiated in future studies.

References

I.Abdul Wadood, Mehreen Ghufran, Syed Babar Jamal, Muhammad Naeem1 , Ajmal Khan2 , Rukhsana Ghaffar3 and Asnad. 2013. Phytochemical Analysis of Medicinal Plants Occurring in Local Area of Mardan. Biochemistry & Analytical Biochemistry. 2(4):2-4.

II.Adegoke, Anthony A. Adebayo-tayo, and Bukola C. 2009. Antibacterial activity and phytochemical analysis of leaf extracts of Lasienthera africanum. African Journal of Biotechnology.8(1): 77-80.

III.Alagesaboopathi, C. 2009. Ethnomedicinal plants and their utilization by villagers in umaragiri hills of salem district of Tamilnadu, India. AfricanJournal ofTraditional Complementary and Alternative Medicines.6: 222-227.

IV.Alagesaboopathi, C. 2011. Ethnobotanical studies on useful plants of kanjamalai hills of salem district of Tamil Nadu, Southern India. Archives of Applied Science Research.3(5): 532-539. 4.

V.Asolkar, L.V., Kakkar, K.K. and Chakre, O.J. 1992. Second supplement to glossary of indian medicinal plants with active principles part I (A-K). (1965-81). CSIR, New Delhi. 18-20.

VI.Ayesha Noor, Gunasekaran, S., Soosai Manickam, A. and Vijayalakshim, M.A. 2008. Antidiabetic activity of Aloe vera and histology of organs in Streptozotocin induced diabetic rats. Current Science. 94: 1070-1076.

VII.Bibitha, B., Jisha, V.K., Salitha, C.V., Mohan, S. and Valsa, A.K. 2002. Antibacterial activity of different plant extracts. Short Communication. Indian Journal of Microbiology. 42: 361-363.

VIII.Bose, T.K., and L.P. Yadav. 1989. Commercial Flowers.Kolkata: Nayaprakash.

IX.Chandra S, Chandra R, Sharma HP, Jha S (2008). Agrobacterium rhizogenes mediated genetic

X.transformations of Spilanthes paniculata (DC.) Jansen. Int J of Plant Sci 3:471-476.

XI.Chung, K.T., Wong, T.Y., Wei, C.I., Huang, Y.W. and Lin, Y.1998. Tannins and human health: a review. Critical reviews in food science and nutrition. 38(6):421-64.

XII.Cragg, G.M. and Newman, D.J. 2001. Medicinal for the millennia. Ann.Ny Academic Science.

XIII.953: 3-25.

XIV.Cowan, M.M. 1999. Clincal Microbiology Revised.12: 564.

XV.Dhasarathan, P., George, L., Ranjitsingh, A.J.A., Padmalatha, C., Madhumitha, M. 2007.Immunomodulation of humoral immune response by Carbosulfan in swiss albino mice. Journal of Current Science.; 10(2): 679-682.

XVI.Dheeba, B., Sampathkumar, P., Sathiya Priya, R.R. and Kannan M. 2010. Phytochemicals studies and evaluation of antioxidant potential of various extracts of Aegle marmelos bark. Pharmacologyonline. 3: 831-839.

XVII.Evans, D.A., Tanis, S.P. and Hart, D.J. 1981. A convergent total synthesis of (±) colchicine and (±) Deacetoamidoisocolchicine. Journal of American Chemistry Society. 103:5813-5821.

XVIII.Geetha, K. 2014.Antimicrobial and phytochemical analysis of Cassia tora(leaves)and Capsicum annum (stalk). Geissman, T.A. 1963. Flavonoid, tannin, lignin compounds, pyrrole pigments,isoprenoid compounds and phenolic plants constitutes.

XIX.Geissman, T.A. 1963. Flavonoid, tannin, lignin compounds, pyrrole pigments,isoprenoid compounds and phenolic plants constitutes.

XX.Han X, Shen T, Lou H (2007). Dietry polyphenols and their biological significance. Int J Mol Sci

XXI.8:950-988.

XXII.Harbone, J.B. 1985. Phytochemical Methods. 2ndedn.Academic Press. London. 309.

XXIII.Haroon, K., Murad, A.K. and Iqbal, H. 2008. Enzyme inhibition activities of the extracts from rhizomes of Gloriosa superba Linn. (Colchicaceae). Journal of enzyme inhibition and medicinal chemistry. 22(6): 722-725.

XXIV.Hemaiswarya S, Raja R, Anbazhagan C, Thiagarajan V (2009). Antimicrobial and mutagenic

XXV.properties of the root tubers of Gloriosa superba Linn. (Kalihari). Pak. J. Bot., 41(1):

XXVI.293-299.

XXVII.Himal Paudel Chhetri, Nisha Shrestha Yogol, Jyoti Sherchan, Anupa, K.C. and Mansoor panna Thapa. S. 2008. Phytochemical and antimicrobial evaluations of some medicinal plants of Nepal. Kathmandu University Journal of science, Engineering and Technology. 1(5): 49-54.

XXVIII.Jana, S. and Shekhawat, G.S. 2011. Critical review on medicinally potent plant species: Gloriosa superba.L. Fitoterapia.82(3):293-301.

XXIX.James Hamuel Doughari. 2012. Phytochemicals: Extraction methods, basic structures and mode of action as potential chemotherapeutic agents. Phytochemicals - A Global perspective of their role in nutrition and health.Technology Europe. 2:538.

XXX.Just MJ, Recio MC, Giner RM, Cueller MU, Manez S, Billia AR and Rios JL (1998).

a. Antiinflammatory activity ofunusual lupine saponins from Bupleurum. Fruticescens,

b. 64: 404-407.

XXXI.Kala, C., Farooquee, N. and Dhar, U. 2004. Prioritization of medicinal plants on the basis of available knowledge, existing practices and use value status in Uttaranchal, India. Biodiversity and Conservation. 13(2): 453-469.

XXXII.Kapoor,L.D. 2001. Tradicinal uses of medicinal plant In: Ayurvedic medicinal plant.CRC press,

XXXIII.New Delhi.

XXXIV.Karuppusamy S (2009). A review on trends in production of secondary metabolites from higher

XXXV.plants by in vitro tissue, cell cultures. J of Med Plant Res 3:1222-1239.

XXXVI.Kee, N.L.A., Mnonopi, N., Davids, H., Naude, R.J. and Frost, C.L. 2008. Antithrombotic / anticoagulant and anticancer activities of selected medicinal plants from South Africa. African Journal of Biotechnology. 7(3): 217-223.

XXXVII.Liu, R.H. 2004. Potential synergy of phytochemicals in cancer prevention: mechanism of action. Journal of Nutrition.134: 3479-3485.

XXXVIII.Maghrani, M., Zeggwah, N., Michel, J. and Eddouks M. 2005. Antihypertensive effect of Lepidium sativum in spontaneously hypertensive rats. Journal of Ethnopharmocology. 102(1-2): 193-197.

XXXIX.Mahesh, B., and Sathish S. 2008. Antimicrobial activity of some important medicinal plant against plant and human pathogens. World Journal ofAgricultural Sciences. 4: 839-843.

XL.Mariappan Senthilkumar. 2013. Phytochemical screening of Gloriosa superba L. from different

a. geographical positions. InternationalJournal of Scientific and research publication.3(1):

b. 2250-3153.

XLI.Marjorie C (1996). Plant products as antimicrobial agents.Clincal Microbiology Review,

XLII.12: 564- 582.

XLIII.Nand P, Drabu S, Gupta RK (2012). Phytochemical and antimicrobial screening of medicinal

a. plants for the treatment of acne. Indian J Nat Prod Res 3(1):28-32.

XLIV.Nattanmai Sundararaman Jeganathan and Rajappan Manavalan. 2013. In vitro antimicrobial activity and phytochemical analysis of Cassia auriculata L. International Current Pharmaceutical Journal.2(6):105-108.

XLV.Ncube, N.S., Afolayan, A.J., and Okoh, A.I. 2008. Assessment techniques of Antimicrobial properties of natural compounds of plant origin: current methods and future trends. African Journal of Biotechnology. 7 (12): 1797- 1806.

XLVI.Nobori T, Miurak K, Wu DJ, Takabayashik LA and CarsonDA (1994). Deletion of cyclin-

a. dependent kinase-4inhibitor gene in multiple human cancers. Nature, 46:753-756.

XLVII.Neuwinger, H.D., 1996. African ethnobotany: Poisons and drugs, chemistry, pharmacology, toxicology. Chapman and Hall, London [translated by the author and Aileen Porter].

XLVIII.Okwu DE (2004). Phytochemicals and vitamin content of indigenous species of south-eastern

XLIX.Nigeria. Journal of Sustainable Agriculture and Environment, 6(1): 30-37.

L.Omale James and Emmanuel.T. Friday. 2010. Phytochemical composition, Bioactivity and

LI.wound healing potential of Euphorbia heterophylla (Euphorbiaceae) leaf extract.

LII.International Journal on Pharmaceutical and Biomedical Research. 1: 54-63.

LIII.Purohit, P. and Bohra, A. 1998. Effect of some plant extracts on conidial germination of some important phytopathogenic fungi. Geobio New Report. 17: 183-184.

LIV.Rebecca Thombre, Rajshree Jagtap and Neeta Patil. 2013Evaluation of phytoconstituents,

LV.antibacterial, antioxidant and cytotoxic activity of Vitex negundo L. and Tabernaemontana

LVI.divaricata L. Int J Pharm Bio Sci; 4(1): 389 – 396.

LVII.Rakesh dadsena, Nitish kumar sahu, shirish agrwal, and anil kumar ,2013 Phytochemical

LVIII.analysis of three endangered plants (Costus specious, Gloriossa superba linn and Rauvolfia

LIX.serpentine (linn) benth) from kanker district of chattisgarh, India. The Bioscan 8(2): 655-659

LX.Ravindra, A. and Mahendra, K. R. 2009. Review: Current advances in Gloriosa superba L.

LXI.Biodiversitas.10(4): 210-214.

LXII.Savithramma, N., Linga Rao, M. and Suhrulatha, D. 2011. Screening of medicinal plants for secondary metabolites. Middle-East Journal of Science Research. 8(3):579-584.

LXIII.Schultes, R.E. 1978. The kingdom of plants, In W.A.R. Thomson (ed.), Medicines from the earth. Mc Graw-Hill Book Co. 208.

LXIV.Sivakumar, G. and Krishnamurthy, K.V. 2002. Gloriosa superba L. - a very useful medicinal plant. In: Recent Progress in Medicinal Plants. USA. 465-82.

LXV.Sodipo, O.A., Akiniyi, J.A. and Ogunbamosu, J.U. 2000. Studies on certain Characteristics of extracts of bark of Pansinystalia macruceras (K schemp) pierre Exbeille. Global Journal of pure applied science. 6: 83-87.

LXVI.Srivastava, U. C. and Chandra, V. 1977. Gloriosa superba Linn. (Kalihari) - An important colchicine producing plant. Journal of Research Indian Medicine.10: 92-95.

LXVII.Srivastava, J., Lambert, J. and Vietmeyer, N. 1996. Medicinal plants: An expanding role in development. World Bank Technical Paper. No.320.

LXVIII.Sundaraganapathy,R.,Niraimathi, V., Ananda Thangadurai, Kamalakannan, D.,Narasimhan, B. and Aakash Deep. 2013.Anti-anxiety activity of Gloriosa superba Linn. Hygeia Journal of Drug and Medicine.5 (1):148 -153.

LXIX.Suresh Kumar, P., Sucheta, S., Sudarshana Deepa, V., Selvamani, P. and S. Latha. 2008. Antioxidant activity in some selected Indian medicinal plants. African Journal of Biotechnology. 7(12): 1826-.1828.

LXX.Swarna priya, R., Doraipandian, A., Arumugam, T., and Radha, N.S. 1995. Floral biology of Gloriosa superba L. South Indian horticulture.43(1-2):40-41.

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2016-04-30

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