PREVALENCE OF EXTENDED-SPECTRUM BETA-LACTAMASE-PRODUCING PATHOGENS FROM URINARY TRACT INFECTED SAMPLES AND THEIR SENSITIVITY PATTERN AGAINST METHANOLIC EXTRACT OF JUGLANS REGIA

Authors

  • Aashaq Hussain Allaie R.P. Mishra Environmental Biotechnology Lab. Department of P.G. Studies & Research in Biological Sciences R. D. University, Jabalpur (M.P)

Keywords:

Abstract

Urinary tract infections (UTI) caused by resistant bacteria are becoming more prevalent. The spread of extended-spectrum beta-lactamase (ESBL)-producing bacteria worldwide has become a serious public health issue among healthcare centers and in the community. Drugs and many secondary metabolites from medicinal plants have proved to be economical and effective against many pathogens.

Infected urine clinical samples were collected aseptically and processed immediately for the isolation of pathogens. Isolated pathogens were identified based on physiological characters and their ESBL pattern was determined by the stroke method. The sensitivity of ESBL strains against Juglans regia was evaluated by the disc diffusion method.

Totally 16 isolates were isolated and 72.5% showed resistance to cefixime, chloramphenicol, clotrimazol, amoxicillin, ampicillin, amikacin and penicillin antibiotics. Of the 26 sample, 61% of the isolates were found to be ESBL producers and highest incidence was found in the age group of 30-45 years. Extended-spectrum beta-lactamase producers belonging to the following species, E. coli, Proteus sp and Pseudomonas aeruginosa, were also multidrug resistant. All three tested ESBL pathogens were highly sensitive to the leaf extracts of Juglans regia and moderately sensitive to root extracts. The maximum zone of inhibition of leaf extracts was 19 ± 0.2 mm against ESBL E. coli followed by 18 ± 0.2 mm against ESBL Pseudomonas aeruginosa and Proteus sp.

The present study reveals the potential role of Juglans regia against UTI pathogens and it confirms the antibacterial activity of this species against drug resistant clinical pathogens.

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Published

2017-06-30

Issue

Vol. 6 No. 7 (2017): June 2017 Special Issue -(D.N.Jain 5th International Conference )11-13 March 2017

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Articles

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