ISOLATION OF MARINE COASTAL MICRO ORGANISMS FROM INDIAN EAST COAST AND ASSESSING THE ENZYME SECRETING POTENTIALS OF SELECTED ISOLATES WITH ITS APPLICATIONS

Abstract

The ocean is a vast space on the earth which has more habitats ranging from micro to macro organisms. There is a rapid growth of microbes in the open sea. However, most of the microbial concentration is seen in the coastal zones than in any other zones of the ocean, as these coastal regions have direct contact with humans and animals. The study describes the various micro organisms isolated from the coastal regions of the Indian East Coast by culturing and sequencing them. Interestingly, most of the isolates are common terrestrial microbes such as Bacillus subtilis, Aspergillusniger,E.coli,Penicillium, Vibrio species. From these selected isolates the enzymatic activities are studied.The common enzymes obtained where Amylase, Protease, Lipase, Invertase, Penicillanase, Keratinase. Severe competition between these microbes have led them to produce various enzymes and to get adapted for variable conditions and changes which had brought in evolution of new species. The study of these microbial enzymes is still a beginning, yet has its own benefits in industrial applications. ;mso-layout-grid-align: none;text-autospace:none'>Totally 16 isolates were isolated and 72.5% showed resistance to cefixime, chloramphenicol, clotrimazol, amoxicillin, ampicillin, amikacin and penicillin antibiotics. Of the 26 sample, 61% of the isolates were found to be ESBL producers and highest incidence was found in the age group of 30-45 years. Extended-spectrum beta-lactamase producers belonging to the following species, E. coli, Proteus sp and Pseudomonas aeruginosa, were also multidrug resistant. All three tested ESBL pathogens were highly sensitive to the leaf extracts of Juglans regia and moderately sensitive to root extracts. The maximum zone of inhibition of leaf extracts was 19 ± 0.2 mm against ESBL E. coli followed by 18 ± 0.2 mm against ESBL Pseudomonas aeruginosa and Proteus sp.The present study reveals the potential role of Juglans regia against UTI pathogens and it confirms the antibacterial activity of this species against drug resistant clinical pathogens.