SENSITIVITY TESTING OF KASTLE MEYER AND LEUCOMALACHITE GREEN AS PRESUMPTIVE TESTS FOR DETECTION OF DRIED BLOOD

Authors

  • Titiksha J. Desai Scientist – Helik Advisory Ltd.

Keywords:

eucomalachite, glucose, environment.

Abstract

To characterize the prevalence of blood contamination on glucose strips used in hospitals, a first of its kind study is in need to be conducted which will recommend monitoring actions to help decrease the risk of spread of infectious agents within hospital environment.  Each glucose strip out of almost 20 vials, each containing 20 glucose strips, will be inspected visually and sampled for testing with Kastle Meyer test and Leucomalachite Green test for hemoglobin to detect the presence of blood. A preliminary study for validating the tests and testing the sensitivity of both these tests was conducted. The sensitivity was determined by testing serial dilutions of whole blood samples in the series of 10-1, 10-2, 10-3, 10-4, 10-5 and 10-6 using 0.9% normal saline as diluents. The whole blood and normal saline were used as positive and negative control for the test. Triplicate experiment was performed on each of the dilutions to validate the two tests. Findings of this study indicate that the Kastle Meyer test is relatively most sensitive.

References

REFERENCES

Louie RF, Lau MJ, Lee JH, Tang Z, Kost GJ. Multicenter study of the prevalence of blood contamination on point-of-care glucose meters and recommendations for controlling contamination. Point of Care 2005; 4: 158–163.

http://www.nfstc.org/pdi/lab_manual/pdi_lab_userguide.pdf

Higaki RS, Philip WMS. A Study of the sensitivity stability and specificity of Phenolphthalein as an indicator test for blood. Can Soc Forens Sci J.1976; 9(3).

Cox M. A Study of the Sensitivity and Specificity of Four Presumptive Tests for Blood. J Forensic Sci. 1991; 36(5): 1503-1511.

Tobe SS, Watson N, Dae´id NN. valuation of Six Presumptive Tests for Blood, Their Specificity, Sensitivity, and Effect on High Molecular-Weight DNA. J Forensic Sci. 2007; 52(1).doi:10.1111/j.1556-4029.2006.00324.

Pérez-Ayala M, Oliver P, Rodríguez Cantalejo F. Prevalence of bacterial contamination of glucose test strips in individual single-use packets versus multiple-use vials. J Diabetes Sci Technol. 2013; 7(4): 854-62.

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Published

2014-08-31

Issue

Vol. 3 No. 9 (2014): AUGUST 2014

Section

Articles

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