IMMUNE-OXIDATIVE ALTERATIONS IN CULTURED HUMAN LYMPHOCYTES BY CIGARETTE SMOKE
Keywords:
Cigarette smoke, Oxidative deregulation, immune response, antioxidant defense enzymes, lymphocytes.Abstract
Cigarette smoke induces numerous disorders and cause inflammatory and airways lungs diseases. The degree of damage by smoking compounds is now serious concern, therefore deciphering the molecular oxidative mechanisms underlying cigarette smoke is one of the major task in modern biology. On this premises we study the oxidative disturbances by cigarette smoke in cultured human lymphocytes to address the molecular upsets. The present study was carried out to assess the immune-oxidative response of cigarette smoke and its mode of action at a molecular level on cultured human lymphocytes isolated from healthy human volunteers. Studies were conducted to evaluate both dose- and time-dependent (n=3) response using cigarette smoke extract (CSE) in vitro. Evaluation of immune-oxidative response by levels of antioxidant defense system enzyme glutathione reductase (GR), superoxide dismutase (SOD) and catalase were carried out. The results of the study showed a time- and dose-dependent response, providing evidence to hitherto unknown oxidative mechanisms of smoking exposure at a genomic level. We envisage our data along with other investigated reports in the literature would help to design better approaches in risk assessment, better diagnostics and therapeutic strategies of practiced and addicted individuals.
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