ANTIMICROBIAL ACTIVITY OF TERMINALIA CHEBULA WITH RESPECT TO COLIFORMS: COMPARISON OF IN VITRO AND IN VIVO GROWN LEAF EXTRACT
Keywords:
Antimicrobial activity, secondary metabolites, explant, micropropagation, coliformsAbstract
The present study deals with the in-vitro micropropagation of Terminalia chebula and evaluation of antimicrobial activity against coliforms. Terminalia chebula (Combretacea) commonly known as haritaki is an important medicinal plant. Explants were cultured on woody plant medium containing various combinations of BAP and NAA for multiple shooting. Woody plant medium supplemented with IBA proved excellent for in-vitro rooting. Antimicrobial activity was evaluated by agar well diffusion method. Methanolic leaf extracts of in-vitro regenerated T.chebula exhibited higher antimicrobial activity as compared to naturally growing plants, against coliforms.
References
Ciocan I D,Bara I I, Plant products as antimicrobial agents, Analele S¸tiint¸ifice ale Universit˘at¸ii, Alexandru IoanCuza, Sect¸iunea Genetic˘a s¸i BiologieMolecular˘a, vol. 8, pp. 151–156, 2007.
Federspil P, Wulkow R, Zimmermann T, (1997) Effect of standardized Myrtol in therapy of acute sinusitis—Results of a double-blind, randomized multicenter study compared with placebos. Laryngo-Rhino-Otologie 76, 23–7
Grzegorczyk I, Matkowski A, Wysokinska H, (2007). Antioxidant activity of extracts from in vitro cultures of Salvia officinalis L. Food Chem. 104(2): 536-541
Khorasani A, Wirakarnain S, Koshy P, Rosna M T, and ArashRafat, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. Accepted 19 October, 2010
Lloyd G, McCown B H, 1981. Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia by shoot tip culture. Proc. Int. Plant Prop. Soc. 30:421-427
Mehta J, Sain M, Mathuriya B, Naruka R, Kavia A and Sharma D, Rapid micropropagation and callus induction of Terminalia bellerica Roxb. - An endangered plant , Asian Journal of Plant Science and Research, 2012, 2 (3): 364-368
Perex CC, Paul M L, Bazerque P 1990. Antibiotic assay by agar well diffusion method. Experimental Medicine and Biology, 15: 113 -115
Rathnamoorthy R, Thilagvathi G, (2014), Characterisation and in-vitro evaluation of Terminalia chebula for antibacterial potential, International journal of pharmacy and pharmaceutical science, 6 (l2).
Srivastav P K,Ranjan P,Sinha S S, CONSERVATION OF TERMINALIA GENETIC RESOURCES: THE PRINCIPAL SOURCE OF NON-WOOD FOREST PRODUCTS IN INDIA, FOREST GENETIC RESOURCES N.24.FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS, 1996
Verpoorte R, Contin A, Memelink J (2002). Biotechnology for the production of plant secondary metabolites. Phytochem. Rev. 1(1):13-25.
VonSchindl R (1972). Inhalative WirkungaetherischerOele. Wiener MedizinischeWochenschrift 122, 591–3
Downloads
Published
Issue
Section
License
Copyright Notice
Submission of an article implies that the work described has not been published previously (except in the form of an abstract or as part of a published lecture or academic thesis), that it is not under consideration for publication elsewhere, that its publication is approved by all authors and tacitly or explicitly by the responsible authorities where the work was carried out, and that, if accepted, will not be published elsewhere in the same form, in English or in any other language, without the written consent of the Publisher. The Editors reserve the right to edit or otherwise alter all contributions, but authors will receive proofs for approval before publication.
Copyrights for articles published in World Scholars journals are retained by the authors, with first publication rights granted to the journal. The journal/publisher is not responsible for subsequent uses of the work. It is the author's responsibility to bring an infringement action if so desired by the author.