IN VITRO CALLUS INDUCTION AND MULTIPLE SHOOT INDUCTION OF OCIMUM TENUIFLORUM
Keywords:
Ocimum, shoot, callus, induction, in vitroAbstract
The present study is focused on tissue culture of Ocimum tenuiflorum collected from Sirsi region Western ghat, using leaves and auxillary bud explants. An efficient protocol for callus initiation and shoot initiation of Ocimum tenuiflorum using leaves and auxillary buds as explants has been established in this work. The tried combinations were BAP (0 to 3.0 mg/l), Kn (0 to 2.5 mg/l) keeping NAA (0.2 mg/l) constant for auxillary bud explants. Various combination of 2,4-D (0 to 1 mg/l), NAA (0 to 1 mg/l) keeping BAP (0.5 mg/l) constant. The maximum number of shoots (7) and shoot length (6 cm) were obtained from combination of 1.5 mg/l BAP, 1 mg/l Kn and 0.2 mg/l NAA. The combination showed 80% survival. The combination of 0.4 mg/l 2.4-D, 0.6 mg/l NAA mg/l and BAP 0.5 mg/l showed maximum callus mass with 55% survival. The combination of 1 mg/l 2, 4-D showed maximum callus was with 40% survival.
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