OIL REGULATORY GENES OF BRASSICA JUNCEA (INDIAN MUSTARD): A REVIEW
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Abstract
Improvement in seed oil content is a major target of plant breeders and biotechnologists worldwide. Import of edible oil in our own country to meet our demand-supply gap cost several thousands of crores in valuable foreign exchange. Therefore development of strategies to increase oil content of oilseed crops is of paramount importance. Brassica species comprise oilseed and a variety of vegetable crops. In the Indian subcontinent, Brassica juncea (Indian mustard) is the major oilseed crop after groundnut.Oil is mostly accumulated in seeds in the form of Triacylglycerols (TAGs). These TAGs are synthesized from fatty acyl-CoA and glycerol-3-phosphate in the endoplasmic reticulum of the cell. Fatty acids are exclusively synthesized in the plastids from acetyl-CoA and then transported in the form of fatty acyl-CoA to the cytoplasm. TAGs are synthesized by the stepwise acylation of glycerol-3-phosphate in the endoplasmic reticulum, (Kennedy pathway). Phosphatidic acid is formed from glycerol-3-phosphate with the help of two enzymes, glycerol-3-phosphate acyltransferase and lyso-phosphatidic acid acyltransferase, which add fatty acyl moieties to the sn-1 and sn-2 positions of glycerol-3-phosphate respectively. Phosphatidic acid is hydrolysed by the enzyme phosphatidate phosphahydrase to give diacylglycerol (DAG). TAGs are synthesized by the addition of a third acyl chain to DAG by the enzyme diacylglycerol acyltransferase.
References
Srivastava A, Gupta V, Pental D, Pradhan AK (2001). AFLP-based genetic diversity assessment amongst agronomically important natural and some newly synthesized lines of Brassica juncea. TheorAppl Genet 102:193–199.
Jagannath A, Sodhi Y.S., Gupta V., Mukhopadhyay A., Arumugam N., Singh I., Rohatgi S., Burma P.K., Pradhan A.K., Pental D. (2011). Eliminating expression of erucic acid-encoding loci allows the identification of ‘‘hidden’’ QTL contributing to oil quality fractions and oil content in Brassica juncea (Indian mustard). TheorAppl Genet 122:1091-1103.
Koornneef, M., Dellaert, L.W. and van der Veen, J.H. (1982). EMS- and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutat. Res. 93, 109–123.
Rerie, W.G., Feldmann, K.A. and Marks, M.D. (1994) The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis. GenesDev. 8, 1388–1399.
Szymanski, D.B., Jilk, R.A., Pollock, S.M. and Marks, M.D. (1998) Control of GL2 expression in Arabidopsis leaves and trichomes. Development, 125, 1161–1171.
Masucci, J.D., Rerie, W.G., Foreman, D.R., Zhang, M., Galway, M.E., Marks, M.D. and Schiefelbein, J.W. (1996). The homeobox gene GLABRA2 is required for position-dependent cell differentiation in the root epidermis of Arabidopsisthaliana. Development, 122, 1253–1260.
Shen, B., Sinkevicius, K.W., Selinger, D.A. and Tarczynski, M.C. (2006) Thehomeobox gene GLABRA2 affects seed oil content in Arabidopsis. PlantMol. Biol. 60, 377–387.
Di Cristina, M., Sessa, G., Dolan, L., Linstead, P., Baima, S., Ruberti, I. and Morelli, G. (1996). The Arabidopsis Athb-10 (GLABRA2) is an HDZip protein required for regulation of root hair development. PlantJ. 10, 393-402.
Western, T.L., Young, D.S., Dean, G.H., Tan, W.L., Samuels, A.L. and Haughn, G.W. (2004) MUCILAGE-MODIFIED4 encodes a putative pectin biosynthetic enzyme developmentally regulated by APETALA2, TRANSPARENTTESTAGLABRA1, and GLABRA2 in the Arabidopsis seed coat. Plant Physiol. 134, 296–306.
Kerstetter, R., Vollbrecht, E., Lowe, B., Veit, B., Yamaguchi, J. and Hake, S. (1994). Sequence analysis and expression patterns divide the maize knotted1-like homeobox genes into two classes. Plant Cell 6, 1877-1887.
Lu, P., Porat, R., Nadeau, J. and O’Neill, S. (1996). Identification of a meristem L1 layer-specific gene in Arabidopsis that is expressed during embryonic pattern formation and defines a new class of homeobox genes. Plant Cell 8, 2155-2168.
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